The incident happened about 9:50 p.m. Friday, the 101st Airborne announced Saturday morning. The crash is under investigation.
The names of the two soldiers will not be released until their families are notified.
The crew was conducting routine training at the time of the accident, according to the 101st Airborne. There were no other casualties.
Fort Campbell Fire and Emergency Services are on site and recovery operations are ongoing, officials said.
“This is a day of sadness for Fort Campbell and the 101st Airborne,” said Brig. Gen. Todd Royar, acting senior commander of the 101st Airborne Division and Fort Campbell, in a statement. “Our thoughts and prayers are with the families during this difficult time.”
Friday’s crash comes on the heels of a difficult few weeks for military aviation.
And on April 4, an F-16 from the Air Force’s Thunderbirds crashed near Nellis Air Force Base, Nevada, killing the pilot.